• 50,000 hours of illumination • Short light path providing better detection of fluorescent signals • Adjustable light intensity for reduced photobleaching • Continuous illumination for consistent results
EVOS™ Light Cubes
      Cell Counting
      Vessel Holders
      Image Capture
      Time Lapse
Demo video
The powerful yet easy-to-use Invitrogen™ EVOS™ FL Cell Imaging System provides the flexibility to fit most epifluorescence microscopy applications. Combining precision components with unique design functionality, the EVOS FL Imaging System has revolutionized fluorescent microscopy.
For Research Use Only. Not for use in diagnostic procedures.
Virtual Demo
Invitrogen EVOS™ FL Cell Imaging System
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Light Cubes
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Because EVOS™ systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days.
Stability comparison. Mercury and metal halide vs. LED
The LED bulbs on the EVOS™ systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb (1,500 hours for a metal halide bulb). That means a 70–75% savings in the overall upkeep of your instrument.
Light CubeDyeCat. No. DAPIDAPI, Hoechst, BFPAMEP4650 TagBFPTagBFPAMEP4668 CFPECFP, Lucifer YellowAMEP4653 GFPGFP, Alexa Fluor™ 488, FITCAMEP4651 RFPRFP, Alexa Fluor™ 555, Alexa Fluor™ 568, Cy™3, MitoTracker™ Orange, Rhodamine Red, DsRedAMEP4652 Texas Red™Texas Red™, Alexa Fluor™ 594, MitoTracker™ Red, mCherry, Cy™3.5AMEP4655 Cy™5Cy™5, Alexa Fluor™ 647, Alexa Fluor™ 660, DRAQ5™AMEP4656 Cy™5.5Cy™5.5, Alexa Fluor™ 680, Alexa Fluor™ 700AMEP4673 Cy™7Cy™7, IRDye 800CWAMEP4667
For a complete list of EVOS™ Light Cubes click here
EVOS™ FL in the cell culture hood
• 15” color, 1024 × 768 pixels; adjustable tilt • Fits in tissue culture hood or screen • 1 DVI digital output for projection display DVI output allows projection onto large screens or monitors making the EVOS FL Imaging System perfect for classroom and teaching environments.
EVOS™ FL in the classroom
Hardware - Monitor
Neuronally differentiated human stem cells (10x),  Light cubes: DAPI, GFP, RFP
Camera specificationsMonochrome camera: Sony ICX445 monochrome CCD, ⅓”, 1280 × 960, 1.3 MegapixelsColor camera: Sony ICX285AQ color CCD, ⅔”, 1360 × 1024, 1.4 Megapixels
Hardware - Camera
Moss antheridial head polytrichum
The EVOS™ FL Imaging System are available with either a monochrome or color camera • Monochrome cameras are commonly used for high-performance fluorescence applications, and provide the best sensitivity for detection of faint fluorescence signals. • Color cameras have lower fluorescence sensitivity but have the advantage of being able to differentiate structures by color in transmitted light (for example, imaging stained tissue samples).
Hardware - Dimensions
EVOS™ FL Imaging System in action
Height: 57.8 cm (22.75 in) Depth: 47.0 cm (18.5 in) Width: 35.5 cm (14.0 in) Weight: 15.3 kg (33.7 lb)
Hardware - Vessel Holders
Easily switch vessel holders for imaging of cells grown in a range of vessels:      • Multiwell plates   • T-flasks      • Petri dishes  • Microscope slides Custom holders also available
For the complete line of EVOS™ vessel holders, click here
• Saved images folder
• Start time lapse button
Software - Time Lapse
• File name input
Click any item below to highlight it on the software panel:
• Image interval input
• File type selection
• Image duration input
The Time Lapse feature allows you program the instrument to record time lapse images.  To create a video (.avi) file, select the Write Video File option prior to running the time lapse session (lower left corner of the Time Lapse Tool bar window)
• Unit measurement selection
• Video file
• Settings control button
  > Find and focus
• Scalebar/Toolbar options
  > Actual—Exposure time slider
• Color option
  > Overlay—Overlay color dialog box
• Image capture button
• Light ON/OFF
• Exposure time slider
• Selected objective
Integrated software is a key component of the all-in-one EVOS FL Imaging System. The intuitive EVOS FL software is designed to enable even the most novice user to capture fluorescent cell images in a matter of minutes.
• Illumination slider
• Channel indicator bar
• Info display bar
Software - Image Capture
• Login button
• Save image button
• Control bar tabs
Click any item below to highlight it on the software panel:
• Show grid
• Total cell count
• Add/delete labels
• Clear all button
• Label input
• Hide tags button
Software - Cell Counting
The Count tool streamlines cell counting by marking items with up to 6 labels onscreen. As you tag items, the system will keep a running tally of counts with percentages for each label assigned. Document your results simply by saving the tagged image, with the Count tool displaying the totals.
• Save image with toolbar button
• Label specific count
Interactive Virtual Demo Explore the innovative features of the EVOS™ FL Cell Imaging System with this virtual demo. Spin the unit around and interact with the key features of the system. Experience how the EVOS FL Cell Imaging System provides the flexibility to fit most epifluorescence microscopy applications. Choose options from the main menu below to read more about how EVOS FL Cell Imaging System is perfect for live cell imaging, area scanning, automated cell counting, image tiling/stitching, and time-lapse imaging. View this on desktop or tablets (best viewed in landscape mode) Click/tap to continue
Objectives - Plan Achromat
• Five position, automated control turret
Plan AchromatObjectives for general application; color and focus with standard correction MagnificationNAWD (mm)BrightfieldPhaseLWDCCOilCat. No. 2x0.065.10• • AMEP4631 4x0.1316.90••• AMEP4632 10x0.256.90••• AMEP4633 20x0.406.80••• AMEP4634 40x0.653.10••• AMEP4635 50x0.950.19• ••AMEPOP050 100x1.250.15• ••AMEPOP100
NA=Numerical aperture, WD=Working Distance, LWD=Long Working Distance, CC=Coverslip corrected
For a complete list of EVOS™ Objectives, please click here
Changing colors is as simple as moving a lever
Light cubes can be easily removed and new light cubes inserted to increase the functionality of the instrument
Plan FluoriteExcellent resolution resulting in brighter fluorescence signal and higher-contrast imaging.Helps reduce optical aberrations; color and focus have a higher level of correction. MagnificationNAWD (mm)BrightfieldPhaseLWDCCOilCat. No. 4x0.1319.70• • AMEP4622 4x0.1316.90••• AMEP4680 10x0.308.30• • AMEP4623 10x0.259.20••• AMEP4681 20x0.457.10• • AMEP4624 20x0.403.10••• AMEP4682 20x0.502.50• • AMEP4698 40x0.652.80• • AMEP4625 40x0.651.60••• AMEP4683 40x0.750.72• • AMEP4699 40x1.300.20• ••AMEP4635 60x0.752.20• • AMEP4626 100x1.280.21• ••AMEP4700
Objectives - Plan Fluorite
Plan FluoriteHighest levels of resolution, fluorescence brightness, contrast, and chromatic correction MagnificationNAWD (mm)BrightfieldPhaseLWDCCOilCat. No. 1.25x0.045.00• • AMEP4736 2x0.086.22• • AMEP4751 4x0.1613.0• • AMEP4752 10x0.403.10• • AMEP4753 20x0.750.60• • AMEP4734 40x0.403.10• • AMEP4753 60x1.420.15• ••AMEP4694 100x1.400.13• ••AMEP4733
Objectives - Plan Apochromat
DVI output allows projection onto large screens or monitors making the EVOS FL Imaging System perfect for classroom and teaching environments.
Hardware - USB / DVI ports